User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/06/20: Difference between revisions
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== | ==Things to do== | ||
* | *Purchase reagents for RNA extraction via Sean's protocol | ||
*Purchase reagents for Northern blotting according to Brian's protocol | |||
*Grow cells | |||
*Lyse (denaturing lysis is fine) | |||
*Can we store samples | |||
*Design and order probes | |||
*Do test Northerns | |||
==Timeline== | |||
*Order reagents today | |||
*Do extractions Tuesday/Wednesday | |||
*Do trial Northern Thursday | |||
*Try polysome stuff the following week | |||
==Questions for Brian== | |||
*Any problems with RNAse when doing the native lysis? | |||
*Protocol for Northerns | |||
*Equipment needed to image gel | |||
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Revision as of 10:38, 20 June 2008
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Things to do
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Questions for Brian
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