User:Audrey Denkler/Notebook/Biology 210 at AU: Difference between revisions
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Plants and Fungi- 2/5/16 | |||
Three of plant samples collected from transect 5 were found within the vegetable bins in our transect, and the other three were found on the ground growing out through the wood chips. Plant one was a bigger green leaf with rough round edges. It had net-like veins throughout and was found within one of the planters in our transect. The second plant was long and skinny. It had small straight pine-like leaves growing off with one singular vein growing through the leaves. This plant was found on the ground in our transect along the fence. The third plant was found on the ground growing through the wood chips in our transect, and had small leaves with wavy cut leaves and a small web-like vascular system. The fourth plant that was found was found growing out of the ground through the wood chips it had very large leaves with very dramatic wavy cuts and a very short small stem. The last plant was found in the planter in our transect and had very small round leaves with a waxy sort of coating on the leaves. Eat stock grows many little leaves with a regular sized stem. The pictures of these plants along with the plant characterization table can be found at this link: audreydenklerbiology210.tumblr.com . | |||
Microbiology Lab- 1/29/16 | Microbiology Lab- 1/29/16 | ||
To gram stain the slides, colonies from a 10^-3 and 10^-7 with and without tetracycline were taken and gram stained. To do this, tiny amounts of the bacterial colonies were scooped using a sterile inoculating loop and mixed into a drop of water on a slide. The slide was then heat fixed the slides by passing them over the flame three times bacteria side up. Then working on the staining tray the slide was covered with crystal violet for one minute, and then rinsed off with water. Next the bacterial smear was covered with Gram's iodine mordant for one minute, and then rinsed off with water. The slide was then decolorized by being flooded with 95% alcohol for 10-20 seconds. It was then gently rinsed. The slide was then covered with a safranin stain for 20-30 seconds, and then rinsed again with water. Next the excess water was blotted off the slide and allowed to air dry. This was repeated for all four variations of our chosen colonies and then examined under a microscope. Those results can be seen in the images at this link: audreydenklerbiology210.tumblr.com . | To gram stain the slides, colonies from a 10^-3 and 10^-7 with and without tetracycline were taken and gram stained. To do this, tiny amounts of the bacterial colonies were scooped using a sterile inoculating loop and mixed into a drop of water on a slide. The slide was then heat fixed the slides by passing them over the flame three times bacteria side up. Then working on the staining tray the slide was covered with crystal violet for one minute, and then rinsed off with water. Next the bacterial smear was covered with Gram's iodine mordant for one minute, and then rinsed off with water. The slide was then decolorized by being flooded with 95% alcohol for 10-20 seconds. It was then gently rinsed. The slide was then covered with a safranin stain for 20-30 seconds, and then rinsed again with water. Next the excess water was blotted off the slide and allowed to air dry. This was repeated for all four variations of our chosen colonies and then examined under a microscope. Those results can be seen in the images at this link: audreydenklerbiology210.tumblr.com . | ||
Revision as of 17:32, 11 February 2016
Plants and Fungi- 2/5/16 Three of plant samples collected from transect 5 were found within the vegetable bins in our transect, and the other three were found on the ground growing out through the wood chips. Plant one was a bigger green leaf with rough round edges. It had net-like veins throughout and was found within one of the planters in our transect. The second plant was long and skinny. It had small straight pine-like leaves growing off with one singular vein growing through the leaves. This plant was found on the ground in our transect along the fence. The third plant was found on the ground growing through the wood chips in our transect, and had small leaves with wavy cut leaves and a small web-like vascular system. The fourth plant that was found was found growing out of the ground through the wood chips it had very large leaves with very dramatic wavy cuts and a very short small stem. The last plant was found in the planter in our transect and had very small round leaves with a waxy sort of coating on the leaves. Eat stock grows many little leaves with a regular sized stem. The pictures of these plants along with the plant characterization table can be found at this link: audreydenklerbiology210.tumblr.com .
Microbiology Lab- 1/29/16
To gram stain the slides, colonies from a 10^-3 and 10^-7 with and without tetracycline were taken and gram stained. To do this, tiny amounts of the bacterial colonies were scooped using a sterile inoculating loop and mixed into a drop of water on a slide. The slide was then heat fixed the slides by passing them over the flame three times bacteria side up. Then working on the staining tray the slide was covered with crystal violet for one minute, and then rinsed off with water. Next the bacterial smear was covered with Gram's iodine mordant for one minute, and then rinsed off with water. The slide was then decolorized by being flooded with 95% alcohol for 10-20 seconds. It was then gently rinsed. The slide was then covered with a safranin stain for 20-30 seconds, and then rinsed again with water. Next the excess water was blotted off the slide and allowed to air dry. This was repeated for all four variations of our chosen colonies and then examined under a microscope. Those results can be seen in the images at this link: audreydenklerbiology210.tumblr.com .
To perform PCR for 16s amplification two PCR tubes were labeled in detail of their contents and group identification. Then 20 µl of a primer/water mixture was added into the labeled PCR tubes. It was then mixed to dissolve the PCR bead. With a sterile toothpick, a small amount of the 10^-3 with and with tetracycline on separate toothpicks was scooped and mixed into their respective labeled PCR tubes. The tubes were then capped and placed in the PCR machine.
Serial Dilution Results Table and Bacteria Characterization Table can also be see at this link: audreydenklerbiology210.tumblr.com .
