User:Asya L. Tucker/Notebook/Asya 571/2015/11/10: Difference between revisions
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Volume of buffer: 1mL - 0.05mL = 0.95mL | Volume of buffer: 1mL - 0.05mL = 0.95mL | ||
==Data== | ==Data== | ||
[[Image:Graph_1nM_Trypsin.Abs_vs_Wl.png|700px]] | |||
[[Image:Graph_1nM_Trypsin.Abs_vs_time_I.png|700px]] | |||
[[Image:Graph_1nM_Trypsin.Abs_vs_time_II.png|700px]] | |||
[[Image:Graph_1nM_Trypsin.Abs_vs_time_III.png|700px]] | |||
[[Image:Graph_1nM_Trypsin.Abs_vs_time_IIII.png|700px]] | |||
==Results== | ==Results== |
Revision as of 07:22, 1 December 2015
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ObjectiveMeasure protease Trypsin's kinetics with the Bradford Assay using 1 nM of protease (Trypsin) at different heating time intervals. ProcedureThe general protocol detailed in Dr. Hartings' notebook was used. The following specific steps were performed:
Calculations: 1st Dilution V1 = [(1µM)(1mL)]/63.52µM = 0.0157mL, amount of trypsin solution needed Volume of buffer: 1mL - 0.0157mL = 0.9843mL 2nd Dilution V1 = [(0.05µM)(1mL)]/1µM = 0.050mL, amount of trypsin solution needed Volume of buffer: 1mL - 0.05mL = 0.95mL DataResults |