User:Asya L. Tucker/Notebook/Asya 571/2015/10/06: Difference between revisions
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==Procedure== | ==Procedure== | ||
*Oceanography | *Oceanography | ||
#Centrifuged falcon tubes containing gold fibers. | #Centrifuged falcon tubes containing gold fibers. (300 rpm for 15 minutes) | ||
#Removed water with plastic pipette. | #Removed water with plastic pipette. | ||
#Prepare the sample that goes in the 3mL cuvette that will be used for ocean optics. | #Prepare the sample that goes in the 3mL cuvette that will be used for ocean optics. | ||
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==Data== | ==Data== | ||
[[Image:Ocean_Optics_TrypsinandAUfibers_at533nm_6Oct.png|700px]] | |||
[[Image:Ocean_Optics_TrypsinandAUfibers_6Oct.png|700px]] | |||
==Results== | ==Results== | ||
Revision as of 12:12, 7 October 2015
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ObjectiveDo the second run on ocean optics to obtain data for trypsin and fibers. Procedure
Calculations for volume of trypsin, and volume of buffer C1·V1 = C2·V2,
where C1 = 66.52µM
C2 = 1µM
V2 = 3mL
V1 = 0.045mL
Volume of buffer: 3mL-0.045mL = 2.955mL
Data
Results | |
