User:Asya L. Tucker/Notebook/Asya 571/2015/09/16: Difference between revisions
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==Procedure== | ==Procedure== | ||
To Prep 1 mL fiber samples | To Prep 1 mL fiber samples | ||
# We centrifuge our samples and removed the supernatant | |||
# We left the tubes open so that rest of the water can evaporate | |||
To prepare a 500 mL of 10 mM Tris buffer at pH 9 | To prepare a 500 mL of 10 mM Tris buffer at pH 9 | ||
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To prepare a 500 mL of 10 mM phosphate buffer at pH 7 | To prepare a 500 mL of 10 mM phosphate buffer at pH 7 | ||
#sodium phosphate monobasic, MM = 137.99 g/mol | |||
#potassium phosphate dibasic: 174.18 g/mol | |||
#Need 0.0005mol of each to make the solution. | #Need 0.0005mol of each to make the solution. | ||
#Weigh 0.398g of sodium phosphate monobasic. Actual amount weighed: 0.40053g | #Weigh 0.398g of sodium phosphate monobasic. Actual amount weighed: 0.40053g |
Revision as of 10:21, 6 October 2015
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Objective
ProcedureTo Prep 1 mL fiber samples
To prepare a 500 mL of 10 mM Tris buffer at pH 9
To prepare a 500 mL of 10 mM phosphate buffer at pH 7
Calculations: Wanted concentration: 10mM = (10-3)M = 10(-3) mol/L Wanted volume: 500mL = 0.5L Number of moles: (10-3 mol/L)/2 = (5-4) mol Amount of solute needed: MM of solute·(5*10-4) mol = amount that should be weighed ResultsThe final pH and conductivity reading for the tris buffer was 9.01 and 293 μS/cm. The final pH and conductivity reading for the phosphate buffer was 6.89 and 14.63 μS/cm. |