User:Asya L. Tucker/Notebook/Asya 571/2015/09/15: Difference between revisions

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## Volume of water: 3,198 μL
## Volume of water: 3,198 μL


 
[[User:Matt Hartings|Matt Hartings]]You need to discuss the results from #1, #2, and #3 from your objectives. What is the data from the tube measurement? What was the result of your measurements with the ocean optics? What were the results of your fiber synthesis?
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Revision as of 17:47, 22 September 2015

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Objective

  1. To prepare gold fibers that we'll will use for the rest of the semester.
  2. To compile all of the groups UV/Vis, florescence, and average eppendorf tube mass data.
  3. To run our Au fibers with trypsin on the Ocean Optics Spec.

Procedures

Preparing gold fiber

  1. We followed the protocol from September 2nd except we used the stock concentration made by Dr. Hartings today, see description.

Eppendorf tube

  1. We weighed out the weight of 15 eppendorf tubes and uploaded the data to dropbox.

Ocean Optics

  1. We measured (Insert data on trypsin gold fibers, buffer and water)


Description

Target Concentrations

Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions.

Stock solutions

  1. HAuCl4·H2O
    1. 2.5 mM
    2. MW = 339.79 g/mol
    3. Volume = 50 mL
    4. 42.4 mg
  2. Lysozyme
    1. 20 μM
    2. MW = 14307 g/mol
    3. Volume = 100 mL
    4. 28.6 mg

Actual Concentrations

The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water.

Stock solutions

  1. HAuCl4·H2O
    1. 50.38 mg
    2. MW = 339.79 g/mol
    3. Volume = 50 mL
    4. 2.96 mM
  2. Lysozyme
    1. 28.69 mg
    2. MW = 14307 g/mol
    3. Volume = 100 mL
    4. 20.1 μM

Directions for making samples

Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers.

  1. 1 mL samples
    1. Volume of gold stock: 84.4 μL
    2. Volume of protein stock: 276 μL
    3. Volume of water: 640 μL
  2. 5 mL samples
    1. Volume of gold stock: 422 μL
    2. Volume of protein stock: 1,380 μL
    3. Volume of water: 3,198 μL

Matt HartingsYou need to discuss the results from #1, #2, and #3 from your objectives. What is the data from the tube measurement? What was the result of your measurements with the ocean optics? What were the results of your fiber synthesis?


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