User:Asya L. Tucker/Notebook/Asya 571/2015/09/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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# The average mass of an eppendorf tube is 1.025135426 and the standard deviation was 0.005373647. | # The average mass of an eppendorf tube is 1.025135426 and the standard deviation was 0.005373647. | ||
# As a class the Lysozyme Molar Absorbtivity Coefficient: 33200 M<sup>-1</sup> cm<sup>-1</sup> and an R<sup>2 equal to 0.9846. | # As a class the Lysozyme Molar Absorbtivity Coefficient: 33200 M<sup>-1</sup> cm<sup>-1</sup> and an R<sup>2 equal to 0.9846. | ||
Latest revision as of 01:09, 27 September 2017
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Objective
Procedures
Ocean Optics Instrument settings - total measurement for 2.5 hours and a reading was recorded ever 2 minutes. Sample preparation
Calculations for volume of trypsin: C1·V1 = C2·V2, where C1 = 52.36µM C2 = 1µM V2 = 3mL V1 = 57.3µL DescriptionTarget Concentrations Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions. Stock solutions
Actual Concentrations The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water. Stock solutions
Directions for making samples Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers.
Data
Results
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