User:Asya L. Tucker/Notebook/Asya 571/2015/09/02: Difference between revisions
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==Procedure== | ==Procedure== | ||
Dr. Hartings left a procedure for us to use in his lab [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2015/09/02| notebook]]. | Dr. Hartings left a procedure for us to use in his lab [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2015/09/02| notebook]] (also listed below). | ||
Target Concentrations | |||
Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions. | |||
Stock solutions | |||
HAuCl4·H2O | |||
2.5 mM | |||
MW = 339.79 g/mol | |||
Volume = 50 mL | |||
42.4 mg | |||
Lysozyme | |||
20 μM | |||
MW = 14307 g/mol | |||
Volume = 100 mL | |||
28.6 mg | |||
Actual Concentrations | |||
The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water. | |||
Stock solutions | |||
HAuCl4·H2O | |||
50.32 mg | |||
MW = 339.79 g/mol | |||
Volume = 50 mL | |||
2.96 mM | |||
Lysozyme | |||
30.65 mg | |||
MW = 14307 g/mol | |||
Volume = 100 mL | |||
21.4 μM | |||
Directions for making samples | |||
Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers. | |||
1 mL samples | |||
Volume of gold stock: 84.4 μL | |||
Volume of protein stock: 259 μL | |||
Volume of water: 656 μL | |||
5 mL samples | |||
Volume of gold stock: 422 μL | |||
Volume of protein stock: 1,297 μL | |||
Volume of water: 3,281 μL | |||
==Data== | ==Data== | ||
Revision as of 09:42, 2 September 2015
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ObjectiveTo make Lysozyme-AuNP fiber for future experiments. ProcedureDr. Hartings left a procedure for us to use in his lab notebook (also listed below). Target Concentrations Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions. Stock solutions HAuCl4·H2O 2.5 mM MW = 339.79 g/mol Volume = 50 mL 42.4 mg Lysozyme 20 μM MW = 14307 g/mol Volume = 100 mL 28.6 mg Actual Concentrations The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water. Stock solutions HAuCl4·H2O 50.32 mg MW = 339.79 g/mol Volume = 50 mL 2.96 mM Lysozyme 30.65 mg MW = 14307 g/mol Volume = 100 mL 21.4 μM Directions for making samples Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers. 1 mL samples Volume of gold stock: 84.4 μL Volume of protein stock: 259 μL Volume of water: 656 μL 5 mL samples Volume of gold stock: 422 μL Volume of protein stock: 1,297 μL Volume of water: 3,281 μL DataNotes | |
