User:Anthony Salvagno/Notebook/Research/2011/05/04/Breaking Down Tethering: Difference between revisions

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(New page: {{qn}}I want to learn more about the tethering process and what each component does. This way if something goes wrong it will be easier to troubleshoot then if I didn't know. I'm sure this...)
 
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Latest revision as of 09:24, 4 May 2011

I want to learn more about the tethering process and what each component does. This way if something goes wrong it will be easier to troubleshoot then if I didn't know. I'm sure this will be an ongoing project.

Components and Properties

  • anti-dig - needed for tethering
  • PBS
  • DNA - required for tethering
  • microspheres - required for tethering
  • BGB - thought to block surface and keep microspheres separated
  • popping buffer - for use with enzymes primarily, but used for generic tethering
    • EDTA - to sequester Mg++ ions
    • NaCl
    • Sodium Phosphate
    • Tween
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