User:Anthony Salvagno/Notebook/Research/2011/01/14/pBSTxi Primer Designing: Difference between revisions
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(New page: {{qn}}I have a 5kb vector and I want to make a 4.4kb PCR product with it. The sequence actually cloned in reverse, so we want the reverse primer to be dig labeled. I am using [http://www.n...) |
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{{qn}}I have a 5kb vector and I want to make a 4.4kb PCR product with it. The sequence actually cloned in reverse, so we want the reverse primer to be dig labeled. I am using [http://www.ncbi.nlm.nih.gov/tools/primer-blast | {{qn}}I have a 5kb vector and I want to make a 4.4kb PCR product with it. The sequence actually cloned in reverse, so we want the reverse primer to be dig labeled. I am using [http://www.ncbi.nlm.nih.gov/tools/primer-blast this site] for primer design. | ||
==Results== | ==Results== |
Revision as of 11:45, 14 January 2011
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I have a 5kb vector and I want to make a 4.4kb PCR product with it. The sequence actually cloned in reverse, so we want the reverse primer to be dig labeled. I am using this site for primer design.