User:Anthony Salvagno/Notebook/Research/2010/12/20/New Anchor PCR Construct Design: Difference between revisions
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==Primers== | ==Primers== | ||
Initial work didn't go so well as Primer3 couldn't find suitable primer pairs, but I think I found a decent pair manually. | |||
Forward Primer: | |||
*GGATCTCAACAGCGGTAAG - located between 701 and 751. It is 19bp long and melts at like 61C. | |||
Reverse Primer: | |||
*CTTCCAGATCGTTGGACAG - located just beyond 201. It too is 19bp long and melts at 61C. | |||
Total product length equals 4087bp. | |||
==Reaction Settings== | ==Reaction Settings== | ||
Latest revision as of 14:40, 21 December 2010
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Primers
Initial work didn't go so well as Primer3 couldn't find suitable primer pairs, but I think I found a decent pair manually.
Forward Primer:
- GGATCTCAACAGCGGTAAG - located between 701 and 751. It is 19bp long and melts at like 61C.
Reverse Primer:
- CTTCCAGATCGTTGGACAG - located just beyond 201. It too is 19bp long and melts at 61C.
Total product length equals 4087bp.
