User:Anthony Salvagno/Notebook/Research/2010/12/15/New Tweezer Construct: Designing the DNA: Difference between revisions
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==The Search for a Plasmid== | ==The Search for a Plasmid== | ||
* | The conditions: | ||
*cheap | |||
*contains exact BstXI cut site from pRL574 | |||
Revision as of 09:28, 15 December 2010
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How should I organize this page? I don't think I currently have a planning category. Should I make one? With that said I'm going to be building a new unzipping/stretching construct. We want to use a new plasmid that we can buy cheaply. Perhaps one with the same BstXI site as pRL574 so that we don't have to redesign the adapter (but I think I want to make that anyway because I want to add a few biotin more to the adapter to enhance microsphere attachment). I will start by finding a plasmid with that site and then design 2 or more PCR reactions (4.4kb reaction and 1.1kb reaction).
The Search for a Plasmid
The conditions:
- cheap
- contains exact BstXI cut site from pRL574
