User:Anthony Salvagno/Notebook/Research/2010/12/15/New Tweezer Construct: Designing the DNA: Difference between revisions
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{{qn}} | {{qn}}How should I organize this page? I don't think I currently have a planning category. Should I make one? With that said I'm going to be building a new unzipping/stretching construct. We want to use a new plasmid that we can buy cheaply. Perhaps one with the same BstXI site as pRL574 so that we don't have to redesign the adapter (but I think I want to make that anyway because I want to add a few biotin more to the adapter to enhance microsphere attachment). I will start by finding a plasmid with that site and then design 2 or more PCR reactions (4.4kb reaction and 1.1kb reaction). | ||
==The Search for a Plasmid== | |||
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Revision as of 09:17, 15 December 2010
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How should I organize this page? I don't think I currently have a planning category. Should I make one? With that said I'm going to be building a new unzipping/stretching construct. We want to use a new plasmid that we can buy cheaply. Perhaps one with the same BstXI site as pRL574 so that we don't have to redesign the adapter (but I think I want to make that anyway because I want to add a few biotin more to the adapter to enhance microsphere attachment). I will start by finding a plasmid with that site and then design 2 or more PCR reactions (4.4kb reaction and 1.1kb reaction).
