User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/11/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Fluorescence Sample Preparation== | ||
The Fluorescence sample was prepared as follows: | |||
[[Image:Calculation temp anneliesefaustino 113016.PNG]] | |||
BSA Fluorescence | |||
# pH 11 | |||
# [Au]=0.25 mM | |||
# [BSA]=3.125 uM | |||
# Vf=3000 uL | |||
Stock Concentrations 161108 | |||
# [BSA]= 30.4 uM | |||
# [Au]= 3.37 mM | |||
# [NaOH]= 1 M | |||
Volumes for making the solution: | |||
# 308.38 uL BSA | |||
# 225.55 uL Au | |||
# 2466.06 uL HOH | |||
# 3.00 uL NaOH | |||
==Data Analysis== | |||
[[Image:Fluorph11emissionamf.PNG]] | |||
This chart shows the emission maximum of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. The emission maximum increases at the very start of the experiment and remains level at around 420 nm for the remainder of the run. No nanoparticles form at pH 11, which is the reason that the emission maximum is relatively stable. | |||
[[Image:Fluorph11integratedamf.PNG]] | |||
This chart shows the integrated intensity of the emission of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. The integrated intensity rapidly increases at the start and then slowly increases over time. | |||
[[Image:Fluorph11scansparta.PNG]] | |||
This chart shows the first few scans of emission intensity of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. As usual, the emission intensity increases rapidly for the first few scans and then does not increase much more. The next two charts show later scans, which remain at about the same emission intensity. | |||
[[Image:Fluorph11scanspartb.PNG]] | |||
[[Image:Fluorph11scanspartc.PNG]] | |||
Latest revision as of 02:10, 27 September 2017
 Project name
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Fluorescence Sample PreparationThe Fluorescence sample was prepared as follows:
BSA Fluorescence
Stock Concentrations 161108
Volumes for making the solution:
Data Analysis
This chart shows the emission maximum of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. The emission maximum increases at the very start of the experiment and remains level at around 420 nm for the remainder of the run. No nanoparticles form at pH 11, which is the reason that the emission maximum is relatively stable.
This chart shows the integrated intensity of the emission of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. The integrated intensity rapidly increases at the start and then slowly increases over time.
This chart shows the first few scans of emission intensity of pH 11, [BSA]=3.125 um, [Au]=0.25 mM. As usual, the emission intensity increases rapidly for the first few scans and then does not increase much more. The next two charts show later scans, which remain at about the same emission intensity.
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