User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==Sample Preparation==
* Insert content here...
 
BSA Fluorescence Solution
# pH 6
# [Au]=0.25 mM
# [BSA]=3.125 uM
# Vf=3000 uL
 
Stock Concentrations 160920
# [BSA]=  52.6 uM
# [Au]= 4.27 mM
# [HCl]= 1mM
 
Volumes for making the solution:
# 3 uL HCl
# 294.6 uL BSA
# 292.7 uL Au
# 2409.5 uL HOH
 
Data was redone by Dr. Hartings as no nanoparticles were formed.
 
<u>3 mL Sample</u>
# 0.25 mM AuCl3
## Stock concentration 4.27 mM
## Volume Added: 176 μL
# 3.125 μM BSA
## Stock Concentration 52.6 μM
## Volume Added: 178 μL
# pH 6
## Stock Concentration 1 mM HCl
## Volume Added: 3 μL
# Volume water added: 2643 μL
 
==Data Analysis==
[[Image:160928redonegraph 1.PNG]]
 
The maximum emission wavelength over time is shown above. The maximum emission spikes after the first time point and remains approximately constant for the rest of the run.
 
[[Image:160928redone graph 2.PNG]]
 
The integrated emission over time is shown above. The integrated emission reaches a maximum around 1000 s. then slowly decreases until 5000 s. until it begins to increase for the rest of the run.
 
[[Image:160928redone graph 3.PNG]]
 
Selected scans in the first 900 seconds are shown. Emission intensity is seen to increase over time.
 
[[Image:160928 redone graph 4.PNG]]
 
Selected scans from 1080-4500 s. are shown. Emission intensity is seen to decrease over this time range.
 
[[Image:160928 redone grpah 5.PNG]]
 
Selected scans from 4680-10800 s. are shown. Emission intensity is seen to slightly increase over this time range.




Latest revision as of 02:02, 27 September 2017

Project name Main project page
Previous entry      Next entry

Sample Preparation

BSA Fluorescence Solution

  1. pH 6
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 52.6 uM
  2. [Au]= 4.27 mM
  3. [HCl]= 1mM

Volumes for making the solution:

  1. 3 uL HCl
  2. 294.6 uL BSA
  3. 292.7 uL Au
  4. 2409.5 uL HOH

Data was redone by Dr. Hartings as no nanoparticles were formed.

3 mL Sample

  1. 0.25 mM AuCl3
    1. Stock concentration 4.27 mM
    2. Volume Added: 176 μL
  2. 3.125 μM BSA
    1. Stock Concentration 52.6 μM
    2. Volume Added: 178 μL
  3. pH 6
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 3 μL
  4. Volume water added: 2643 μL

Data Analysis

The maximum emission wavelength over time is shown above. The maximum emission spikes after the first time point and remains approximately constant for the rest of the run.

The integrated emission over time is shown above. The integrated emission reaches a maximum around 1000 s. then slowly decreases until 5000 s. until it begins to increase for the rest of the run.

Selected scans in the first 900 seconds are shown. Emission intensity is seen to increase over time.

Selected scans from 1080-4500 s. are shown. Emission intensity is seen to decrease over this time range.

Selected scans from 4680-10800 s. are shown. Emission intensity is seen to slightly increase over this time range.



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