User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/27: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==BSA Solution==
==BSA Solution==


BSA Ocean Optics Solution
#BSA Ocean Optics Solution
pH 6
## pH 6
[Au]=0.25 mM
## [Au]=0.25 mM
[BSA]=3.125 uM
## [BSA]=3.125 uM
Vf=3000 uL
## Vf=3000 uL
# Stock Concentrations 160927
## [BSA]= 52.6 uM
## [Au]= 4.27 mM
## [HCl]= 1mM
# Volumes for making the solution:
## 3 uL HCl
## 294.6 uL BSA
## 292.7 uL Au
## 2409.5 uL HOH


Stock Concentrations 160927
==Results==
[BSA]= 52.6 uM
 
[Au]= 4.27 mM
[[Image:OO pH6 part1 160928.PNG]]
[HCl]= 1mM
 
The chart above shows a dramatic increase in maximum wavelength from about ~450 to ~550 at about ~3200s.  
 
[[Image:OO pH6 part3 160928.PNG]]
 
The chart above shows a slight decrease in max absorbance until about 1200 sec. at which point it dramatically increases for the rest of the run.  
 
[[Image:OO pH6 part2 160928.PNG]]
 
The chart above shows a slight decrease in A(535) until about 1200 sec. at which point it dramatically increases for the rest of the run. This is similar to the maximum absorbance over time chart as expected.


Volumes for making the solution:
3 uL HCl
294.6 uL BSA
292.7 uL Au
2409.5 uL HOH


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 02:03, 27 September 2017

Project name Main project page
Previous entry      Next entry

BSA Solution

  1. BSA Ocean Optics Solution
    1. pH 6
    2. [Au]=0.25 mM
    3. [BSA]=3.125 uM
    4. Vf=3000 uL
  2. Stock Concentrations 160927
    1. [BSA]= 52.6 uM
    2. [Au]= 4.27 mM
    3. [HCl]= 1mM
  3. Volumes for making the solution:
    1. 3 uL HCl
    2. 294.6 uL BSA
    3. 292.7 uL Au
    4. 2409.5 uL HOH

Results

The chart above shows a dramatic increase in maximum wavelength from about ~450 to ~550 at about ~3200s.

The chart above shows a slight decrease in max absorbance until about 1200 sec. at which point it dramatically increases for the rest of the run.

The chart above shows a slight decrease in A(535) until about 1200 sec. at which point it dramatically increases for the rest of the run. This is similar to the maximum absorbance over time chart as expected.



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