User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/23/Autoclave
From OpenWetWare
I am concerned about our autoclave. Below is an image of it.
Is this dirty? It looks like we have oxidized copper build up some how. This doesn't make sense since the chamber of the autoclave is stainless steel. Where did this come from? Could copper be contaminating my tools which is leading to problems with our kinesin assays?
SJK 13:33, 24 October 2009 (EDT)
Good point. How much copper would be in the steam? I have no idea, but would have though it'd be small. There is copper in the fill reservoir, but I don't know why there's so much buildup. Chamber-brite may clean it up. However, that wouldn't mean there still isn't trace copper in the steam. But I feel like there wouldn't be much copper in steam...any idea how to calculate that?
Also: check the manual to see if it indicates that we did something wrong, perhaps? Such as letting reservoir get too low or too high?
