User:Andrew W Long/Notebook/Synthesis of Lipid Modulators/2015/10/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 19: Line 19:
MeOH Fraction: [[Image:Awl_meoh_fraction_100515.pdf|250px]]  
MeOH Fraction: [[Image:Awl_meoh_fraction_100515.pdf|250px]]  


The EtOAc fraction shows a clear peak at 287, which is a predicted mass for the desired product (the molar weight + a sodium ion). A likely next step is to run a TLC on the compound in an attempt at further purification.
The EtOAc fraction shows a clear peak at 287 (mass plus H) and 108.8 (mass plus Na). A likely next step is to run a TLC on the compound in an attempt at further purification.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 01:14, 27 September 2017

Project name Main project page
Previous entry      Next entry

Purification of Large-Scale Reaction Between CNP & MTT

The procedure for the reaction being discussed can be found here.

Procedure:

  1. The product of the reaction was put on air for the weekend, and today was redissolved in ethyl acetate.
  2. Another dirty column was prepped using the previous procedure, and both an ethyl acetate and a methanol fraction were collected.
  3. Mass spectrometry was used to analyze the products of the purification. The positive scans are shown below (negative showed nothing important):

EtOAc Fraction: File:Awl etoac fraction 100515.pdf

MeOH Fraction: File:Awl meoh fraction 100515.pdf

The EtOAc fraction shows a clear peak at 287 (mass plus H) and 108.8 (mass plus Na). A likely next step is to run a TLC on the compound in an attempt at further purification.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Andrew_W_Long/Notebook/Synthesis_of_Lipid_Modulators/2015/10/05&oldid=1017362"