User:Andrew W Long/Notebook/Protein Modification/2014/12/01: Difference between revisions
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**No protein+no KCl | **No protein+no KCl | ||
For this next reaction and all reactions to follow, the concentration of OPD was modified. A solution of 1 mg OPD per 1 ml solvent was produced. 0.2025 ml of that were taken, and placed in 1.8225 ml of solvent. That dilute solution was then used for the reaction in the Ocean Optics. | For this next reaction and all reactions to follow, the concentration of OPD was modified. This was done with the hopes of developing a Michaelis-Menten curve. A solution of 1 mg OPD per 1 ml solvent was produced. 0.2025 ml of that were taken, and placed in 1.8225 ml of solvent. That dilute solution was then used for the reaction in the Ocean Optics. | ||
*Pentanol | *Pentanol | ||
**KCl + protein in Phosphate | **KCl + protein in Phosphate | ||
Revision as of 11:12, 1 December 2014
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1-PentanolReactions were run using procedure outlined on Nov 21 2014 Done Today:
For this next reaction and all reactions to follow, the concentration of OPD was modified. This was done with the hopes of developing a Michaelis-Menten curve. A solution of 1 mg OPD per 1 ml solvent was produced. 0.2025 ml of that were taken, and placed in 1.8225 ml of solvent. That dilute solution was then used for the reaction in the Ocean Optics.
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