User:Andrew W Long/Notebook/Protein Modification/2014/06/19: Difference between revisions
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*Once it has run, save it as a .spc file within the program | *Once it has run, save it as a .spc file within the program | ||
*After each run, the cuvette was thoroughly rinsed with distilled water, and completely dried. Excess water was removed with a glass pipette | *After each run, the cuvette was thoroughly rinsed with distilled water, and completely dried. Excess water was removed with a glass pipette | ||
*When done, hit disconnect and turn off | |||
'''Results of Dialysis:''' | '''Results of Dialysis:''' | ||
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*Spectrophotometry revealed for the pH 2.5 sample that, although a large amount of protein was lost, protein was still present in the solution | *Spectrophotometry revealed for the pH 2.5 sample that, although a large amount of protein was lost, protein was still present in the solution | ||
*Similar results were observed for the pH 3.0 sample | *Similar results were observed for the pH 3.0 sample | ||
** | **Slightly more protein was present in the less acidic solution however | ||
*Since so much protein is being lost during dialysis, we will try to use a desalting column to slowly raise the pH | *Since so much protein is being lost during dialysis, we will try to use a desalting column to slowly raise the pH | ||
''' | '''Extraction Using 80 μL 1M HCl:''' | ||
*Same procedures previously described were used, and similar results were obtained | *Same procedures previously described were used, and similar results were obtained | ||
** | **Observable differences: | ||
'''Lyophilizer:''' | '''Lyophilizer:''' |
Revision as of 07:16, 19 June 2014
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June 19, 2014Data Analysis:
UV Vis Initial Setup Instructions
Results of Dialysis:
Extraction Using 80 μL 1M HCl:
Lyophilizer: |