User:Andrew W Long/Notebook/Protein Modification/2014/06/18: Difference between revisions
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==June 18, 2014== | ==June 18, 2014== | ||
'''Overview''' | '''Overview:''' | ||
Solutions that were dialyzed were checked using the spectrophotometer, and it was determined that nearly all protein that was extracted and run through the column was lost during dialysis. Today we are restarting the experiment, and plan to do a few things differently to try to minimize the loss (try manipulating pH, using more dilute myoglobin/buffer solutions, and use different methods/materials for the dialysis). | Solutions that were dialyzed were checked using the spectrophotometer, and it was determined that nearly all protein that was extracted and run through the column was lost during dialysis. Today we are restarting the experiment, and plan to do a few things differently to try to minimize the loss (try manipulating pH, using more dilute myoglobin/buffer solutions, and use different methods/materials for the dialysis). | ||
Revision as of 09:13, 18 June 2014
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June 18, 2014Overview: Solutions that were dialyzed were checked using the spectrophotometer, and it was determined that nearly all protein that was extracted and run through the column was lost during dialysis. Today we are restarting the experiment, and plan to do a few things differently to try to minimize the loss (try manipulating pH, using more dilute myoglobin/buffer solutions, and use different methods/materials for the dialysis). Initial setup:
pH 2.5 Extraction:
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