User:Andrew K Farag/Notebook/CHEM-581/2014/10/08: Difference between revisions

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==Objective==
==Objective==
*Make stock solutions of R6G. Thanks to [[User:Madeleine_Y._Bee/Notebook/CHEM-581_Experimental_Chemistry/2014/10/01|Madeleine]] for making initial 2500μM solution of R6G.
*Make stock solutions of R6G. Thanks to [[User:Madeleine_Y._Bee/Notebook/CHEM-581_Experimental_Chemistry/2014/10/01|Madeleine]] for making initial 2500μM solution of R6G.

Revision as of 15:29, 20 October 2014

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Objective

  • Make stock solutions of R6G. Thanks to Madeleine for making initial 2500μM solution of R6G.
  • Take UV-Vis and Fluorescence spectra of prepared R6G samples
  • Make a calibration curve using both fluorescence and UV-Vis data
  • To finish film synthesis

Notes

The completion of the film synthesis used the procedure from Tami's Notebook

R6G Fluorescence: Calibration and Measurement Completed by Michael

  1. Make stock concentrations (both groups can use the same solutions)
    • Initial stock: 2500μM, secondary stock: 500μM
    • 0.10μM
      • Made with 500μM solution
    • 0.50μM
      • Made with 500μM solution
    • 1.0μM
      • Made with 500uM solution
    • 1.5uM
      • Made with 500μM solution
    • 2.0μM
      • Made with 2500μM solution
    • 0.75μM
      • Made with 500μM solution
    • 1.2μM
      • Made with 500μM solution


Fluorimeter Settings

  • 500nm excitation
  • 515-700nm scan range
  • 10.0nm slit width
  • 200nm/min scan speed