User:Andrew K Farag/Notebook/CHEM-571/2014/03/26

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Objective

  • Repeat the 78% BSA-L-Cysteine Synthesis
  • Continue precipitation of large volume samples

Stock Solution Prep

The solutions created for 78%BSA by mass with L-Cysteine synthesis:

  1. 250mL of 20mM L-cysteine
    1. FW 121.16g/mol
    2. measure out: 0.6058g
    3. measured: 0.6072g
    4. actual conc: 20.05mM
  2. 50mL of 100mM ZnSO4
    1. FW 287.56g/mol
    2. measure out: 1.4378g
    3. measured: 1.4399 g
    4. actual conc: 100.1 mM
  3. 50mL of 10mM MnCl2
    1. FW 197.9g/mol
    2. measure out: 0.09895g
    3. measured: 0.0987g
    4. actual conc: 9.98 mM
  4. 10mL 1M NaOH
    1. FW 40.00g/mol
    2. 0.4284g
    3. Final concentration a bit over 1M check pH carefully when adjusting!
  5. 25mL 100mM Na2S
    1. FW 240.18g/mol
    2. measure out: 0.600g
    3. measured: 0.610g
    4. actual conc: 101.6 mM
  6. 25mL .1484 mM BSA

The protocol followed was the one used previously for the 78% BSA-L-Cysteine QDs

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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