User:Andrew K Farag/Notebook/CHEM-490/2014/09/08: Difference between revisions
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==Description== | ==Description== | ||
I started working this semester, Fall 2014, under the supervision of [[User:Matt Hartings|Dr. Hartings]], with [[User:James C. Schwabacher|James]] and [[User:Eleni N. Kalivas|Eleni]] on Quantum Dots again. The project was started last semester in Chemistry-571, however not enough results were collected. This semester we will be working on synthesizing QDs again using Mercury instead. The procedure is according to [http://www.ncbi.nlm.nih.gov/pubmed/22826036|Protein-directed synthesis of NIR-emitting, tunable HgS quantum dots and their applications in metal-ion sensing.]. | I started working this semester, Fall 2014, under the supervision of [[User:Matt Hartings|Dr. Hartings]], with [[User:James C. Schwabacher|James]] and [[User:Eleni N. Kalivas|Eleni]] on Quantum Dots again. The project was started last semester in Chemistry-571, however not enough results were collected. This semester we will be working on synthesizing QDs again using Mercury instead. The procedure is according to [http://www.ncbi.nlm.nih.gov/pubmed/22826036|Protein-directed synthesis of NIR-emitting, tunable HgS quantum dots and their applications in metal-ion sensing.]. | ||
==Procedure== | |||
*5mL of 5mM HgNO3--HgNO3 was not fully disolving so HgCl2 was used instead | |||
*5mL BSA (15mg/mL) | |||
*final pH around 9 (adjusted by 1M NaOH)--The pH was around 10 in this reaction. Not enough calculations were taken when adding the NaOH. | |||
*The variac was set on 55/100, temperature is around 115C | |||
*vigorous stirring | |||
*stir for 8-12 hours, till color is pale yellow | |||
*Eleni followed the same procedure, however her solution was left in room temperature | |||
*The nitrogen gas and the water supply under the hood was all set for the reflux reaction. | |||
==Calculations== | |||
*HgCl2 in 50 mL of water | |||
*271.5(g/mol)*0.005(mol/L)*0.05L=0.067875g | |||
*BSA in 50 mL of water | |||
*50mL*15(mg/mL)*1(g/1000mg)=0.75g | |||
*NaOH | |||
*40(g/mol)*1(mol/L)*0.05L=2g of NaOH pellets. | |||
--1.9905 g were used |
Revision as of 15:42, 16 September 2014
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DescriptionI started working this semester, Fall 2014, under the supervision of Dr. Hartings, with James and Eleni on Quantum Dots again. The project was started last semester in Chemistry-571, however not enough results were collected. This semester we will be working on synthesizing QDs again using Mercury instead. The procedure is according to synthesis of NIR-emitting, tunable HgS quantum dots and their applications in metal-ion sensing.. Procedure
Calculations
--1.9905 g were used |