User:Andrew K Farag/Notebook/Andrewfarag/2013/10/16

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Current revision (13:48, 22 October 2013) (view source)
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==Entry title==
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objective==
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We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol
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==Description==
we took the UV-vis of HRP from the previous day after it was diluted, 10% HRP and 90% water. We took UV-vis of Cirtrate from august 28. It was already diluted, 10% citrate and 90% citrate.  
we took the UV-vis of HRP from the previous day after it was diluted, 10% HRP and 90% water. We took UV-vis of Cirtrate from august 28. It was already diluted, 10% citrate and 90% citrate.  
<br> A 2.0 ml solution of luminol, hydrogen peroxide and buffer is prepared. Initial concentration of luminol is 1.46 mM. Initial concentration of hydrogen peroxide is 44.29. Final concentration of luminol, as told by Dr. Hartings, should be 0.126 mM, and concentration of hydrogen peroxide is 50 times luminols, 6.3 mM. Accordingly, 0.17 mL of luminol and 0.28 mL of hydrogen peroxide are added to 1.55 mL of the buffer to make the 2ml solution.
<br> A 2.0 ml solution of luminol, hydrogen peroxide and buffer is prepared. Initial concentration of luminol is 1.46 mM. Initial concentration of hydrogen peroxide is 44.29. Final concentration of luminol, as told by Dr. Hartings, should be 0.126 mM, and concentration of hydrogen peroxide is 50 times luminols, 6.3 mM. Accordingly, 0.17 mL of luminol and 0.28 mL of hydrogen peroxide are added to 1.55 mL of the buffer to make the 2ml solution.
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<br> another solution of concentration ratio 90 Au:1 BSA is prepared. Concentration of gold is 2.33 mM. Concentration of BSA is 15 uM. 1.3 mL of gold and 2.24 mL of BSA are added to 6.46 ml of water.  
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<br> another solution of concentration ratio 90 Au:1 BSA is prepared. Concentration of gold is 2.33 mM. Concentration of BSA is 15 uM. 1.3 mL of gold and 2.24 mL of BSA are added to 6.46 ml of water.
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==Data==
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<u>Stock Solution</u>
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# Buffer
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## 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
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# Luminol
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## Dissolve 12.9mg luminol in 300uL of DMSO
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## Add to 50mL buffer ---> 1.46mM
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# H2O2
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## 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
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## Check absorption at 250 [http://www.bio.net/mm/methods/1995-January/023533.html source] (ε(250) = 16.69 M<sup>-1</sup>cm<sup>-1</sup>). A = 0.7392. [H2O2] = 44.29mM
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==Notes==
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This area is for any observations or conclusions that you would like to note.
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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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[[Category:Course]]
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[[Category:Miscellaneous]]
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[[Category:Course]]
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[[Category:Miscellaneous]]
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Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol

Description

we took the UV-vis of HRP from the previous day after it was diluted, 10% HRP and 90% water. We took UV-vis of Cirtrate from august 28. It was already diluted, 10% citrate and 90% citrate.
A 2.0 ml solution of luminol, hydrogen peroxide and buffer is prepared. Initial concentration of luminol is 1.46 mM. Initial concentration of hydrogen peroxide is 44.29. Final concentration of luminol, as told by Dr. Hartings, should be 0.126 mM, and concentration of hydrogen peroxide is 50 times luminols, 6.3 mM. Accordingly, 0.17 mL of luminol and 0.28 mL of hydrogen peroxide are added to 1.55 mL of the buffer to make the 2ml solution.
another solution of concentration ratio 90 Au:1 BSA is prepared. Concentration of gold is 2.33 mM. Concentration of BSA is 15 uM. 1.3 mL of gold and 2.24 mL of BSA are added to 6.46 ml of water.

Data

Stock Solution

  1. Buffer
    1. 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
  2. Luminol
    1. Dissolve 12.9mg luminol in 300uL of DMSO
    2. Add to 50mL buffer ---> 1.46mM
  3. H2O2
    1. 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
    2. Check absorption at 250 source (ε(250) = 16.69 M-1cm-1). A = 0.7392. [H2O2] = 44.29mM

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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