User:Amirah

Initial ideas

• Build a self assembling and degradable scaffold
  • Self assembly would get rid of the use of extraneous material to form proper bonds.
  • If the material is degrabale, it can degrade leaving the scaffold behind.
• Find coating to cover degradable scaffold that can select for viruses or cells that excrete scaffold material (collagen, calcuim bicarbonate etc.)
• Find coating to bind proteins needed to assemble outer scaffold layer.
  • Coating must not alter assembly or binding of scaffold
  • Coating must be very selective for protein or cells needed to form outer non-degradable layer of scaffolding.

Experimental Implementation ideas

• Use DNA as scaffold.
  • DNA is degradable
  • DNA has the ability to be self assembled into 2D structures (Rothemund), and 3D structures (Zhang)
• Build 3D structures from adaptaion of 'raster fill method' (Rothemund)

Module 4 tables

	Surface display of scFv fusion?		Binding to gold?
	Glucose	Galactose	Glucose	Galactose
pCT-CON	N	Y	N	N
pAu1	N	Y	N	Y