User:Amie S. Krupnick/Notebook/Biology 210 at AU: Difference between revisions

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(New page: Procedure I: The Volvocine Line Characteristic Chlamydomonas Gonium Volvox Number of Cells 1 12 1000 Colony Size (um) 10 30 1000 Specialization of Cells No No Yes Mechanisms of Motility ...)
 
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Procedure I: The Volvocine Line
'''Biological Transect: Observing a Niche at American University  January 26, 2015'''


Characteristic Chlamydomonas Gonium Volvox
'''Purpose'''
Number of Cells 1 12 1000
The purpose of this experiment was to observe a 20x20 transect at AU for the Biology-210 lab.  This experiment and the corresponding set of observations will act as the basis for future experiments, using the samples collected during this initial inspection of the transect. After taking a sample of abiotic and biotic components from the transect, the plot of land was discussed in class. This lab was purely observational and did not yield any findings. A Hay Infusion was created with the collected sample to aid in the following week's lab.
Colony Size (um) 10 30 1000
Specialization of Cells No No Yes
Mechanisms of Motility Flagella Flagella No
Isogamous or Oogamous? Isogamous Isogamous Oogamous


'''Materials and Methods'''
In this observational experiment, each group was given a flashlight and a sterile 50 ml conical tube for sample collection representative of the transect.  After being dropped off at the appropriate transect, the 20x20 plot of land was examined and drawn for reference.  The transect is located in the gardens between Hughes Hall and Bender Arena and a concrete pathway running from north to south.  The allotted plastic tube was filled with dirt, soil and vegetation from the transect.  The transect was drawn, with directions labeled, as pictured below.  Upon return to the lab, a Hay Infusion Culture was made from the collected sample.  10 grams of soil and vegetation from the conical tube sample was placed in a plastic jar containing 500 ml of deer park water.  0.1 g of dried milk was added into the jar and the jar was mixed.  After being labeled, the jar was set down without a lid for a week.


Procedure II: Observing a Niche at AU
'''Data and Observations'''
This transect is located on an uneven plot of land that contained many different forms of vegetation.  The vegetation found was largely evenly distributed across the transect. The biotic organisms specified are: trees, leaves, bushes, vegetation and shrubs.  The abiotic components specified are: snow, dirt, hay, wood chips and paper. During the making of the Hay Infusion Culture, dried milk was added as a source of food for the vegetation in the culture. 


My transect is about a 20x20 plot of land located in the garden between Hughes Hall and Bender Arena. The transect is mostly shrubbery with a cement path running down the middle, from north to south.  In terms topography, both abiotic and biotic features were observed.  Biotic life consisted of: trees, leaves, bushes, vegetation and shrubs.  Abiotic occurrences consisted of: snow, dirt, hay, wood chips and paper. The majority of the abiotic and biotic components were found evenly distributed across the transect.  The snow was located on the east side of the transect and the paper was found in the north east corner.
'''Conclusion'''
The jar was set in the back of the lab without a lid for examination of protists and to "inoculate agar petri plates for studying bacteria" (Bentley et al 2015) in the following week.
 
 
 
 
 
 
 
 
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Revision as of 14:41, 26 January 2015

Biological Transect: Observing a Niche at American University January 26, 2015

Purpose The purpose of this experiment was to observe a 20x20 transect at AU for the Biology-210 lab. This experiment and the corresponding set of observations will act as the basis for future experiments, using the samples collected during this initial inspection of the transect. After taking a sample of abiotic and biotic components from the transect, the plot of land was discussed in class. This lab was purely observational and did not yield any findings. A Hay Infusion was created with the collected sample to aid in the following week's lab.

Materials and Methods In this observational experiment, each group was given a flashlight and a sterile 50 ml conical tube for sample collection representative of the transect. After being dropped off at the appropriate transect, the 20x20 plot of land was examined and drawn for reference. The transect is located in the gardens between Hughes Hall and Bender Arena and a concrete pathway running from north to south. The allotted plastic tube was filled with dirt, soil and vegetation from the transect. The transect was drawn, with directions labeled, as pictured below. Upon return to the lab, a Hay Infusion Culture was made from the collected sample. 10 grams of soil and vegetation from the conical tube sample was placed in a plastic jar containing 500 ml of deer park water. 0.1 g of dried milk was added into the jar and the jar was mixed. After being labeled, the jar was set down without a lid for a week.

Data and Observations This transect is located on an uneven plot of land that contained many different forms of vegetation. The vegetation found was largely evenly distributed across the transect. The biotic organisms specified are: trees, leaves, bushes, vegetation and shrubs. The abiotic components specified are: snow, dirt, hay, wood chips and paper. During the making of the Hay Infusion Culture, dried milk was added as a source of food for the vegetation in the culture.

Conclusion The jar was set in the back of the lab without a lid for examination of protists and to "inoculate agar petri plates for studying bacteria" (Bentley et al 2015) in the following week.





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