User:Allison K. Alix/Notebook/Thesis Research/2013/12/17

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December 17th, 2013 Main project page
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1) Prepare 100mM DTT in 4% TEA

From 5/29:

Original DTT concentration 1M

1M(x) = (100x10-3M)(1mL)

x = 0.1mL = 100μL in 900μL 4% TEA

4%(900μL) = 36μL TEA in 864μL PBS

OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS



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