User:Allison K. Alix/Notebook/Thesis Research/2013/12/17

From OpenWetWare

< User:Allison K. Alix | Notebook | Thesis Research | 2013 | 12(Difference between revisions)
Jump to: navigation, search
Current revision (11:51, 17 December 2013) (view source)
 
Line 11: Line 11:
Original DTT concentration 1M
Original DTT concentration 1M
-
1M(x) = (100x10-3M)(1mL)
+
 
 +
1M(x) = (100x10<sup>-3</sup>M)(1mL)
 +
 
x = 0.1mL = 100μL in 900μL 4% TEA
x = 0.1mL = 100μL in 900μL 4% TEA
 +
4%(900μL) = 36μL TEA in 864μL PBS
4%(900μL) = 36μL TEA in 864μL PBS
 +
OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS
OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS

Current revision

December 17th, 2013 Main project page
Previous entry      Next entry

1) Prepare 100mM DTT in 4% TEA

From 5/29:

Original DTT concentration 1M

1M(x) = (100x10-3M)(1mL)

x = 0.1mL = 100μL in 900μL 4% TEA

4%(900μL) = 36μL TEA in 864μL PBS

OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS



Personal tools