User:Allison K. Alix/Notebook/Thesis Research/2013/12/16: Difference between revisions
| (2 intermediate revisions by the same user not shown) | |||
| Line 26: | Line 26: | ||
==DNA Attachment== | ==DNA Attachment== | ||
original [AuNP] = 23.5nM | |||
original [DNA] 63.1uM | |||
attach DNA to AuNP in 75:1 ratio | |||
Final concentrations should be 881.25nM DNA and 11.75nM AuNP | |||
1) Prepare 250uL 3.525uM thiol-DNA | |||
63.1uM (x) = 250uL(3.525uM) | |||
x = 13.97uL 63.1 uM thiol-DNA in 236.03uL 1X PBS | |||
2) React 10 minutes and extract DNA using ethyl acetate | |||
3) Mix DNA with sodium citrate to change pH (THIS MUST BE DONE PRIOR TO ADDING AUNP!!!) | |||
268mM sodium citate buffer | |||
268mM (x) = 10mM(500uL) | |||
x = 18.7uL | |||
500uL-18.7uL = 481.3uL | |||
481.3uL/2 = 240.65uL each of AuNP and DNA | |||
4) Add 240.65uL AuNP | |||
5) React 10 minutes | |||
6) Centrifuge for 4 minutes at 7000rpm | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 11:26, 16 December 2013
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
NEW Telomeric DNAthiol-8 repeat GTTAGGG Tm=69.2°C MW=19,007.5 38.2OD= 62.9nm = 1.20mg 1.20mg x mol/19,007.5g x 1g/1000mg = 6.31x10-8mol dilute in 1mL 1X PBS 6.31x10-8mol / 1mL x 1000mL/1L = 6.31x10-5M 6.31x10-5M x 106μM/1M = 63.1μM DNA Attachmentoriginal [AuNP] = 23.5nM original [DNA] 63.1uM attach DNA to AuNP in 75:1 ratio Final concentrations should be 881.25nM DNA and 11.75nM AuNP 1) Prepare 250uL 3.525uM thiol-DNA 63.1uM (x) = 250uL(3.525uM) x = 13.97uL 63.1 uM thiol-DNA in 236.03uL 1X PBS 2) React 10 minutes and extract DNA using ethyl acetate 3) Mix DNA with sodium citrate to change pH (THIS MUST BE DONE PRIOR TO ADDING AUNP!!!) 268mM sodium citate buffer 268mM (x) = 10mM(500uL) x = 18.7uL 500uL-18.7uL = 481.3uL 481.3uL/2 = 240.65uL each of AuNP and DNA 4) Add 240.65uL AuNP 5) React 10 minutes 6) Centrifuge for 4 minutes at 7000rpm | |
