User:Allison K. Alix/Notebook/Thesis Research/2013/11/19: Difference between revisions
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4) Cool to room temperature | 4) Cool to room temperature | ||
==Notes== | |||
The purpose of this analysis is to observe if fluorescence changes with changing concentrations of Au/DNA. Where as before one Au/DNA/MB solution was prepared and subsequent dilutions were made from that one solution, this procedure first prepares the dilutions and attaches the MB to each separate solution. The higher concentrations of Au/DNA have more binding sites for the beacon, so they are expected to bind fully. The lower concentrations should also bind fully with some closed MB in solution. | |||
Revision as of 08:55, 20 November 2013
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To Do
Sample Preparation1) Prepare the following dilutions of AuNP/DNA: original concentration of AuNP: 290pM 20X 14.5pM 40X 7.25pM 60X 4.83pM 80X 3.625pM 100X 2.9pM 120X 2.4167pM 140X 2.07pM 160X 1.8125pM 180X 1.61pM 200X 1.45pM 240X 1.208pM
3) Heat at ~78°C for 30 minutes 4) Cool to room temperature NotesThe purpose of this analysis is to observe if fluorescence changes with changing concentrations of Au/DNA. Where as before one Au/DNA/MB solution was prepared and subsequent dilutions were made from that one solution, this procedure first prepares the dilutions and attaches the MB to each separate solution. The higher concentrations of Au/DNA have more binding sites for the beacon, so they are expected to bind fully. The lower concentrations should also bind fully with some closed MB in solution.
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