User:Allison K. Alix/Notebook/Thesis Research/2013/11/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> November 5th, 2013</span> | ||
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==Addition of MB== | ==Addition of MB== | ||
1) Add 6uL 7.61uM MB to 50uL AuNP/DNA | 1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution | ||
NEW Concentrations: | |||
MB: 815nM | |||
DNA: 586nM | |||
AuNP: 0.29nM | |||
2) Heat for 25 min at ~76.0°C | |||
3) Run FCS Measurements. Begin with pure sample. Because the AuNP concentration is so low (290pM) it might be hard to get signal from anything lower. | |||
==Other Notes== | |||
The UV-Vis spectra from the beacon alone did not yield the expected results. Not only were the spectra out of order (expecting to see highest concentration have the highest absorbance) but it was jagged, and not smooth like ti normally is. This might be attributed to the fact that the samples were not freshly made (prepared 10/30) and the DNA could have either clumped together causing inaccurate scattering patterns of light or settled to the bottom. (see image below) | |||
[[Image:OWW_MB_uv.png]] | |||
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Revision as of 13:08, 5 November 2013
 November 5th, 2013
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To Do
Data
Calculating AuNP/DNA ConcentrationAuNP: abs @ 532 = 0.03 ε = 92,382,878 c= A/εb c = 3.2473x10-10M = 0.325nM DNA: abs @ 254 = 0.399 ε = 607,500 c = 6.5679E-07M = 656.6nM Addition of MB1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution NEW Concentrations: MB: 815nM DNA: 586nM AuNP: 0.29nM 2) Heat for 25 min at ~76.0°C 3) Run FCS Measurements. Begin with pure sample. Because the AuNP concentration is so low (290pM) it might be hard to get signal from anything lower. Other NotesThe UV-Vis spectra from the beacon alone did not yield the expected results. Not only were the spectra out of order (expecting to see highest concentration have the highest absorbance) but it was jagged, and not smooth like ti normally is. This might be attributed to the fact that the samples were not freshly made (prepared 10/30) and the DNA could have either clumped together causing inaccurate scattering patterns of light or settled to the bottom. (see image below)
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