# User:Allison K. Alix/Notebook/Thesis Research/2013/11/05

(Difference between revisions)
 Revision as of 15:11, 5 November 2013 (view source) (→Addition of MB)← Previous diff Revision as of 15:49, 5 November 2013 (view source) (→Addition of MB)Next diff → Line 35: Line 35: 1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution 1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution + + NEW Concentrations: + + MB: 815nM + + DNA: 2) Heat for 25 min at ~76.0°C 2) Heat for 25 min at ~76.0°C

## Revision as of 15:49, 5 November 2013

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## To Do

• Run UV-Vis of MB samples to determine concentraiton
• Add MB to AuNP/DNA Sample. Run FCS.

## Calculating AuNP/DNA Concentration

AuNP: abs @ 532 = 0.03

ε = 92,382,878

c= A/εb

c = 3.2473x10-10M = 0.325nM

DNA: abs @ 254 = 0.399

ε = 607,500

c = 6.5679E-07M = 656.6nM

## Addition of MB

1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution

NEW Concentrations:

MB: 815nM

DNA:

2) Heat for 25 min at ~76.0°C

3) Prepare 40X-110X dilutions

4) Run FCS Measurements