User:Allison K. Alix/Notebook/Thesis Research/2013/11/05

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(Addition of MB)
(Addition of MB)
Line 34: Line 34:
==Addition of MB==
==Addition of MB==
-
1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solutio
+
1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution
 +
 
 +
2) Heat for 25 min at ~76.0°C
 +
 
 +
3) Prepare 40X-110X dilutions

Revision as of 13:32, 5 November 2013

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To Do

  • Run UV-Vis of MB samples to determine concentraiton
  • Add MB to AuNP/DNA Sample. Run FCS.

Data

Image:OWW_AUNP_DNA_pic.png

Calculating AuNP/DNA Concentration

AuNP: abs @ 532 = 0.03

ε = 92,382,878

c= A/εb

c = 3.2473x10-10M = 0.325nM

DNA: abs @ 254 = 0.399

ε = 607,500

c = 6.5679E-07M = 656.6nM

Addition of MB

1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution

2) Heat for 25 min at ~76.0°C

3) Prepare 40X-110X dilutions



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