User:Allison K. Alix/Notebook/Thesis Research/2013/10/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> October 30th, 2013</span> | ||
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* Prepare MB samples and obtain UV-Vis Spectra. Calculate the concentration of each. | * Prepare MB samples and obtain UV-Vis Spectra. Calculate the concentration of each. | ||
* Attach DNA to AuNP (no silica coating) obtain concentration of DNA attached to AuNP, attach MB. | |||
==Sample Preparation== | ==Sample Preparation== | ||
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22.4nM, 17.9nM, 14.9nM, 12.8nM, 11.2nM, 9.9nM, 8.9nM, and 8.2nM | 22.4nM, 17.9nM, 14.9nM, 12.8nM, 11.2nM, 9.9nM, 8.9nM, and 8.2nM | ||
Original MB concentration: 7.61uM | |||
Dilute to 895nM | |||
7.61x10<sup>-6</sup>M (x) = 895x10<sup>-9</sup>M(75uL) | |||
x = 8.8uL in 66.2uL PBS | |||
Revision as of 13:09, 5 November 2013
 October 30th, 2013
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To Do
Sample PreparationPrepare 150uL of the following MB samples with the following concentrations: 22.4nM, 17.9nM, 14.9nM, 12.8nM, 11.2nM, 9.9nM, 8.9nM, and 8.2nM Original MB concentration: 7.61uM Dilute to 895nM 7.61x10-6M (x) = 895x10-9M(75uL) x = 8.8uL in 66.2uL PBS
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