User:Allison K. Alix/Notebook/Thesis Research/2013/10/29: Difference between revisions
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(Autocreate 2013/10/29 Entry for User:Allison_K._Alix/Notebook/Thesis_Research) |
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== | ==To Do== | ||
* | |||
* Calculate percentage of MB open using FCS curves | |||
* Run 10000X green beads to obtain average diffusion time. | |||
==Calculating %MB open== | |||
Example: | |||
N = 7.9 particles | |||
7.9 particles x 1 mol/6.02x10<sup>23</sup> particles x 1/6.93 x 10<sup>-16</sup>L = 1.89x10<sup>-8</sup>M | |||
**6.93x10<sup>-16</sup> is the focal volume of the spot, previous calculated** | |||
1.89x10<sup>-8</sup>M x 1nM/10<sup>-9</sup>M = 18.9nM | |||
Actual concentration of this sample was 22.4nM (According to dilution calculations) | |||
%MB open = 18.9nM/22.4nM x 100% = 84.4nM | |||
Revision as of 07:13, 30 October 2013
 Project name
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To Do
Calculating %MB openExample: N = 7.9 particles 7.9 particles x 1 mol/6.02x1023 particles x 1/6.93 x 10-16L = 1.89x10-8M
1.89x10-8M x 1nM/10-9M = 18.9nM Actual concentration of this sample was 22.4nM (According to dilution calculations) %MB open = 18.9nM/22.4nM x 100% = 84.4nM
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