User:Allison K. Alix/Notebook/Thesis Research/2013/10/29: Difference between revisions
(→Data) |
(→Data) |
||
Line 37: | Line 37: | ||
The above image is an FCS curve showing the average diffusion time of 9 10000X diluted green bead samples. τ<sub>D</sub> was calculated to be 58.7ms | The above image is an FCS curve showing the average diffusion time of 9 10000X diluted green bead samples. τ<sub>D</sub> was calculated to be 58.7ms | ||
==Calculations== | |||
τD = (ω023ηπd)/4kT | |||
where τD is the diffusion time of the sample | |||
ω0 is the radius of the spot | |||
η is the viscosity of the solvent (water), 0.001028kg/m s | |||
d is the diameter of the particle | |||
k is Boltzmann's constant = 1.3806488 × 10-23 m2 kg s-2-2 K-1 | |||
T is the temperature of the room (18.7 °C, 291.7K) | |||
Revision as of 08:42, 30 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
To Do
Calculating %MB openExample: N = 7.9 particles 7.9 particles x 1 mol/6.02x1023 particles x 1/6.93 x 10-16L = 1.89x10-8M NOTE: 6.93x10-16 is the focal volume of the spot, previous calculated 1.89x10-8M x 1nM/10-9M = 18.9nM Actual concentration of this sample was 22.4nM (According to dilution calculations) %MB open = 18.9nM/22.4nM x 100% = 84.4nM
DataThe above image is an FCS curve showing the average diffusion time of 9 10000X diluted green bead samples. τD was calculated to be 58.7ms CalculationsτD = (ω023ηπd)/4kT where τD is the diffusion time of the sample ω0 is the radius of the spot η is the viscosity of the solvent (water), 0.001028kg/m s d is the diameter of the particle k is Boltzmann's constant = 1.3806488 × 10-23 m2 kg s-2-2 K-1 T is the temperature of the room (18.7 °C, 291.7K)
|