User:Allison K. Alix/Notebook/Thesis Research/2013/10/29: Difference between revisions
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[[Image:Green_bead_igor_curve.png]] | [[Image:Green_bead_igor_curve.png]] | ||
The above image is an FCS curve showing the average diffusion time of 9 10000X diluted green bead samples. τ<sub>D</sub> was calculated to be | The above image is an FCS curve showing the average diffusion time of 9 10000X diluted green bead samples. τ<sub>D</sub> was calculated to be 58.7ms | ||
Revision as of 08:40, 30 October 2013
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To Do
Calculating %MB openExample: N = 7.9 particles 7.9 particles x 1 mol/6.02x1023 particles x 1/6.93 x 10-16L = 1.89x10-8M NOTE: 6.93x10-16 is the focal volume of the spot, previous calculated 1.89x10-8M x 1nM/10-9M = 18.9nM Actual concentration of this sample was 22.4nM (According to dilution calculations) %MB open = 18.9nM/22.4nM x 100% = 84.4nM
DataThe above image is an FCS curve showing the average diffusion time of 9 10000X diluted green bead samples. τD was calculated to be 58.7ms
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