User:Allison K. Alix/Notebook/Thesis Research/2013/10/09

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(Autocreate 2013/10/09 Entry for User:Allison_K._Alix/Notebook/Thesis_Research)
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==Entry title==
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==To Do==
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* Insert content here...
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* There is concern about whether or not the fluorescence we are observing is from the MB bound to the DNA. MB only samples which were at the same concentration as the MB in the Au@SiO2-DNA/MB samples still showed some fluorescence, even though theoretically it should be very low, due to the fact that the beacon is closed. To test whether or not the DNA and MB are bound, we are preparing solutions with varying DNA:MB ratios.  
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==Preparing new Au@SiO2-DNA/MB solutions==
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1) 1:3 DNA:MB
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Original [DNA] = 910nM
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Original [MB] = 7610nM
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MB should be 2730nM
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*Added 20uL 7610nM MB to 25uL Au@Si/DNA solution
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NEW CONCENTRATIONS:
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MB = 3.182uM
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DNA = 404uM
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(This is actually a 1:8 ratio....)
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2) 1:1
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Added 3.3uL 7.61uM MB to 25uL Au@Si/DNA solution
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[MB] = 887nM
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[DNA] = 803nM
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3) 3:1 DNA:MB
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Added 1uL MB to 25uL Au@Si/DNA solution
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[MB] = 292.7nM
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[DNA] = 875nM
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Revision as of 10:18, 9 October 2013

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To Do

  • There is concern about whether or not the fluorescence we are observing is from the MB bound to the DNA. MB only samples which were at the same concentration as the MB in the Au@SiO2-DNA/MB samples still showed some fluorescence, even though theoretically it should be very low, due to the fact that the beacon is closed. To test whether or not the DNA and MB are bound, we are preparing solutions with varying DNA:MB ratios.

Preparing new Au@SiO2-DNA/MB solutions

1) 1:3 DNA:MB

Original [DNA] = 910nM

Original [MB] = 7610nM

MB should be 2730nM

  • Added 20uL 7610nM MB to 25uL Au@Si/DNA solution

NEW CONCENTRATIONS:

MB = 3.182uM

DNA = 404uM

(This is actually a 1:8 ratio....)

2) 1:1

Added 3.3uL 7.61uM MB to 25uL Au@Si/DNA solution

[MB] = 887nM

[DNA] = 803nM

3) 3:1 DNA:MB

Added 1uL MB to 25uL Au@Si/DNA solution

[MB] = 292.7nM

[DNA] = 875nM








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