User:Allison K. Alix/Notebook/Thesis Research/2013/10/09: Difference between revisions
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* There is concern about whether or not the fluorescence we are observing is from the MB bound to the DNA. MB only samples which were at the same concentration as the MB in the Au@SiO2-DNA/MB samples still showed some fluorescence, even though theoretically it should be very low, due to the fact that the beacon is closed. To test whether or not the DNA and MB are bound, we are preparing solutions with varying DNA:MB ratios. | |||
==Preparing new Au@SiO2-DNA/MB solutions== | |||
1) 1:3 DNA:MB | |||
Original [DNA] = 910nM | |||
Original [MB] = 7610nM | |||
MB should be 2730nM | |||
*Added 20uL 7610nM MB to 25uL Au@Si/DNA solution | |||
NEW CONCENTRATIONS: | |||
MB = 3.182uM | |||
DNA = 404uM | |||
(This is actually a 1:8 ratio....) | |||
2) 1:1 | |||
Added 3.3uL 7.61uM MB to 25uL Au@Si/DNA solution | |||
[MB] = 887nM | |||
[DNA] = 803nM | |||
3) 3:1 DNA:MB | |||
Added 1uL MB to 25uL Au@Si/DNA solution | |||
[MB] = 292.7nM | |||
[DNA] = 875nM | |||
Revision as of 08:18, 9 October 2013
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To Do
Preparing new Au@SiO2-DNA/MB solutions1) 1:3 DNA:MB Original [DNA] = 910nM Original [MB] = 7610nM MB should be 2730nM
NEW CONCENTRATIONS: MB = 3.182uM DNA = 404uM (This is actually a 1:8 ratio....) 2) 1:1 Added 3.3uL 7.61uM MB to 25uL Au@Si/DNA solution [MB] = 887nM [DNA] = 803nM 3) 3:1 DNA:MB Added 1uL MB to 25uL Au@Si/DNA solution [MB] = 292.7nM [DNA] = 875nM
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