User:Allison K. Alix/Notebook/Thesis Research/2013/10/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 29: Line 29:


'''ω<sub>0</sub> = 0.549um'''
'''ω<sub>0</sub> = 0.549um'''
* diameter was calculated for all samples with an average of 17.8 nm (not including the diameter calculated from the 61.33pM sample)





Revision as of 11:30, 8 October 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Calculations

τD = (ω023ηπd)/4kT

where τD is the diffusion time of the sample

ω0 is the radius of the spot

η is the viscosity of the solvent (water), 0.001028kg/m s

d is the diameter of the particle

k is Boltzmann's constant = 1.3806488 × 10-23 m2 kg s-2-2 K-1

T is the temperature of the room (18.7 °C, 291.7K)

Calculating ω0 using 10000X diluted green fluorescent beads

average τD = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12)

sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m2 kg s-2 K-1)(291.7K)]/[3(0.001028kg/m s)(pi)(2x10-7) = 5.49x10-7 m

ω0 = 0.549um

  • diameter was calculated for all samples with an average of 17.8 nm (not including the diameter calculated from the 61.33pM sample)