User:Allison K. Alix/Notebook/Thesis Research/2013/10/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> October 8th, 2013</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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T is the temperature of the room (18.7 °C, 291.7K) | T is the temperature of the room (18.7 °C, 291.7K) | ||
==Calculating ω<sub>0</sub> | ==Calculating ω<sub>0</sub> using 10000X diluted green fluorescent beads== | ||
average τ<sub>D</sub> = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12) | average τ<sub>D</sub> = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12) | ||
sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m<sup>2</sup> kg s<sup>-2</sup> | sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m<sup>2</sup> kg s<sup>-2</sup> K<sup>-1</sup>)(291.7K)]/[3(0.001028kg/m s)(pi)(2x10<sup>-7</sup>) = 5.49x10<sup>-7</sup> m | ||
'''ω<sub>0</sub> = 0.549um''' | |||
* diameter was calculated for all samples with an average of 17.8 nm (not including the diameter calculated from the 61.33pM sample) | |||
==FCS measurements of MB only== | |||
prepare the MB at the following concentrations: | |||
Latest revision as of 23:26, 26 September 2017
October 8th, 2013 | Main project page Previous entry Next entry |
CalculationsτD = (ω023ηπd)/4kT where τD is the diffusion time of the sample ω0 is the radius of the spot η is the viscosity of the solvent (water), 0.001028kg/m s d is the diameter of the particle k is Boltzmann's constant = 1.3806488 × 10-23 m2 kg s-2-2 K-1 T is the temperature of the room (18.7 °C, 291.7K) Calculating ω0 using 10000X diluted green fluorescent beadsaverage τD = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12) sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m2 kg s-2 K-1)(291.7K)]/[3(0.001028kg/m s)(pi)(2x10-7) = 5.49x10-7 m ω0 = 0.549um
FCS measurements of MB onlyprepare the MB at the following concentrations:
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