User:Allison K. Alix/Notebook/Thesis Research/2013/10/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> October 8th, 2013</span> | ||
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* diameter was calculated for all samples with an average of 17.8 nm (not including the diameter calculated from the 61.33pM sample) | * diameter was calculated for all samples with an average of 17.8 nm (not including the diameter calculated from the 61.33pM sample) | ||
==FCS measurements of MB only== | |||
prepare the MB at the following concentrations: | |||
Revision as of 13:17, 5 November 2013
 October 8th, 2013
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CalculationsτD = (ω023ηπd)/4kT where τD is the diffusion time of the sample ω0 is the radius of the spot η is the viscosity of the solvent (water), 0.001028kg/m s d is the diameter of the particle k is Boltzmann's constant = 1.3806488 × 10-23 m2 kg s-2-2 K-1 T is the temperature of the room (18.7 °C, 291.7K) Calculating ω0 using 10000X diluted green fluorescent beadsaverage τD = 36.3137ms = 0.0363137 sec (taken from 10000X beads on 9/4, 9/11, and 9/12) sqr rt [(0.0363137s)(4)(1.3806488 × 10-23 m2 kg s-2 K-1)(291.7K)]/[3(0.001028kg/m s)(pi)(2x10-7) = 5.49x10-7 m ω0 = 0.549um
FCS measurements of MB onlyprepare the MB at the following concentrations:
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