User:Allison K. Alix/Notebook/Thesis Research/2013/09/24: Difference between revisions
Line 73: | Line 73: | ||
2) Extract DTT with 4 2mL aliquots of ethyl acetate | 2) Extract DTT with 4 2mL aliquots of ethyl acetate | ||
3) Prepare 100uL 12.5nM AuNP | |||
23.5(x) = 12.5(100uL) | |||
x = 53.2uL in 46.8uL water | |||
4) mix 20uL DNA with 20uL AuNP and 5uL 268mM sodium citrate. React 10 minutes. | |||
5) Centrifuge 7 minutes at 10000rpm. | |||
Revision as of 08:42, 24 September 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
Calculating [AuNP] in Au@SiO2/DNA-MB solutions
1) Addition of 5uL APS (1mL)(5nM)=(1.005mL)(x) x = 4.975nM 2) Addition of 1.5mL Sulfo-SMCC (1.005mL)(4.975nM) = (x)(2.505mL) x = 1.996nM 3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer (1.996nM)(2.505mL) = x(0.530mL) x = 9.43nM 4) Addition of 270uL DNA (9.43nM)(530uL)=x(800uL) x = 6.25nM 5) Addition of 13.3uL 7.61uM MB to 100uL Au@SiO2/DNA 6.25nM(100uL)=x(113.3uL) x = 5.52nM
Preparation of Au/DNA/MB w/o Si for FCSFinal Concentrations need to be [Au] = 6.25nM [DNA] = 910nM 1) Mix 145.9uL 3.64uM thiol-DNA with 145.9uL 100mM DTT in 4% TEA. React 10 minutes 53.1uM(10uL) = 3.64uM(x) 10uL in 135.9 water 2) Extract DTT with 4 2mL aliquots of ethyl acetate 3) Prepare 100uL 12.5nM AuNP 23.5(x) = 12.5(100uL) x = 53.2uL in 46.8uL water 4) mix 20uL DNA with 20uL AuNP and 5uL 268mM sodium citrate. React 10 minutes. 5) Centrifuge 7 minutes at 10000rpm.
|