User:Allison K. Alix/Notebook/Thesis Research/2013/09/24: Difference between revisions
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== | ==Calculating [AuNP] in Au@SiO2/DNA-MB solutions== | ||
* | |||
*Original [Au@SiO2] = 5nM before attaching DNA | |||
1) Addition of 5uL APS | |||
(1mL)(5nM)=(1.005mL)(x) | |||
x = 4.975nM | |||
2) Addition of 1.5mL Sulfo-SMCC | |||
(1.005mL)(4.975nM) = (x)(2.505mL) | |||
x = 1.996nM | |||
3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer | |||
(1.996nM)(2.505mL) = x(0.530mL) | |||
x = 9.43nM | |||
4) Addition of 270uL DNA | |||
(9.43nM)(530uL)=x(800uL) | |||
x = 6.25nM | |||
Revision as of 07:18, 24 September 2013
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Calculating [AuNP] in Au@SiO2/DNA-MB solutions
1) Addition of 5uL APS (1mL)(5nM)=(1.005mL)(x) x = 4.975nM 2) Addition of 1.5mL Sulfo-SMCC (1.005mL)(4.975nM) = (x)(2.505mL) x = 1.996nM 3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer (1.996nM)(2.505mL) = x(0.530mL) x = 9.43nM 4) Addition of 270uL DNA (9.43nM)(530uL)=x(800uL) x = 6.25nM
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