User:Allison K. Alix/Notebook/Thesis Research/2013/09/24: Difference between revisions
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x = 5.52nM | x = 5.52nM | ||
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! Dilution Factor !! [Au@SiO<sub>2</sub>] !! [DNA] !! [MB] | |||
|- | |||
| None (stock) || 5.52nM || 802nM || 895nM | |||
|- | |||
| 2X || 2.76nM || 401nM || 447.5nM | |||
|- | |||
| 10X || 552pM || 80.2nM || 89.5nM | |||
|- | |||
| 100X || 55.2pM || 8.02nM || 8.95nM | |||
|- | |||
| 1000X || 5.52pM || 802pM || 895pM | |||
|- | |||
| 10000X || 552fM || 80.2pM || 89.5pM | |||
|} | |||
Revision as of 07:39, 24 September 2013
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Calculating [AuNP] in Au@SiO2/DNA-MB solutions
1) Addition of 5uL APS (1mL)(5nM)=(1.005mL)(x) x = 4.975nM 2) Addition of 1.5mL Sulfo-SMCC (1.005mL)(4.975nM) = (x)(2.505mL) x = 1.996nM 3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer (1.996nM)(2.505mL) = x(0.530mL) x = 9.43nM 4) Addition of 270uL DNA (9.43nM)(530uL)=x(800uL) x = 6.25nM 5) Addition of 13.3uL 7.61uM MB to 100uL Au@SiO2/DNA 6.25nM(100uL)=x(113.3uL) x = 5.52nM
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