User:Allison K. Alix/Notebook/Thesis Research/2013/09/24: Difference between revisions
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==Preparation of Au/DNA/MB w/o Si for FCS== | |||
Final Concentrations need to be | |||
[Au] = 5.52nM | |||
[DNA] = 802nM | |||
Revision as of 07:41, 24 September 2013
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Calculating [AuNP] in Au@SiO2/DNA-MB solutions
1) Addition of 5uL APS (1mL)(5nM)=(1.005mL)(x) x = 4.975nM 2) Addition of 1.5mL Sulfo-SMCC (1.005mL)(4.975nM) = (x)(2.505mL) x = 1.996nM 3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer (1.996nM)(2.505mL) = x(0.530mL) x = 9.43nM 4) Addition of 270uL DNA (9.43nM)(530uL)=x(800uL) x = 6.25nM 5) Addition of 13.3uL 7.61uM MB to 100uL Au@SiO2/DNA 6.25nM(100uL)=x(113.3uL) x = 5.52nM
Preparation of Au/DNA/MB w/o Si for FCSFinal Concentrations need to be [Au] = 5.52nM [DNA] = 802nM
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