# User:Allison K. Alix/Notebook/Thesis Research/2013/09/24

(Difference between revisions)
 Revision as of 09:14, 24 September 2013 (view source) (Autocreate 2013/09/24 Entry for User:Allison_K._Alix/Notebook/Thesis_Research)← Previous diff Revision as of 09:18, 24 September 2013 (view source) (→Entry title)Next diff → Line 6: Line 6: | colspan="2"| | colspan="2"| - ==Entry title== + ==Calculating [AuNP] in Au@SiO2/DNA-MB solutions== - * Insert content here... + + *Original [Au@SiO2] = 5nM before attaching DNA + + 1) Addition of 5uL APS + + (1mL)(5nM)=(1.005mL)(x) + + x = 4.975nM + + 2) Addition of 1.5mL Sulfo-SMCC + + (1.005mL)(4.975nM) = (x)(2.505mL) + + x = 1.996nM + + 3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer + + (1.996nM)(2.505mL) = x(0.530mL) + + x = 9.43nM + + 4) Addition of 270uL DNA + + (9.43nM)(530uL)=x(800uL) + + x = 6.25nM + + + +

## Revision as of 09:18, 24 September 2013

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## Calculating [AuNP] in Au@SiO2/DNA-MB solutions

• Original [Au@SiO2] = 5nM before attaching DNA

1) Addition of 5uL APS

(1mL)(5nM)=(1.005mL)(x)

x = 4.975nM

2) Addition of 1.5mL Sulfo-SMCC

(1.005mL)(4.975nM) = (x)(2.505mL)

x = 1.996nM

3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer

(1.996nM)(2.505mL) = x(0.530mL)

x = 9.43nM

4) Addition of 270uL DNA

(9.43nM)(530uL)=x(800uL)

x = 6.25nM