User:Allison K. Alix/Notebook/Thesis Research/2013/09/17: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 26: | Line 26: | ||
==Attaching MB to Au@SiO2-DNA== | ==Attaching MB to Au@SiO2-DNA== | ||
1) Add 13.3 uL 7.61uM MB to Au@SiO2-DNA | 1) Add 13.3 uL 7.61uM MB to 100uL Au@SiO2-DNA | ||
2) Hybridize by heating for 20 min @ 75C | 2) Hybridize by heating for 20 min @ 75C | ||
Line 34: | Line 34: | ||
4) Prepare 5X, 10X, and 100X dilutions in PBS | 4) Prepare 5X, 10X, and 100X dilutions in PBS | ||
5) Run FCS Measurements on each. If signal is seen with the least concentrated solution, prepare 1000X and 10,000X dilutions as well. | 5) Run FCS Measurements on each. If signal is seen with the least concentrated solution, prepare 1000X and 10,000X dilutions as well. | ||
==FCS Curves Au@SiO2-DNA-MB== | ==FCS Curves Au@SiO2-DNA-MB== |
Latest revision as of 23:19, 26 September 2017
Project name | Main project page Previous entry Next entry |
To Do
FCS Curves Antibody-Alexa 532Attaching MB to Au@SiO2-DNA1) Add 13.3 uL 7.61uM MB to 100uL Au@SiO2-DNA 2) Hybridize by heating for 20 min @ 75C 3) Slowly cool to room temperature. 4) Prepare 5X, 10X, and 100X dilutions in PBS 5) Run FCS Measurements on each. If signal is seen with the least concentrated solution, prepare 1000X and 10,000X dilutions as well. FCS Curves Au@SiO2-DNA-MBNext Up-Run Au/DNA-MB without silica coating -Run Au@Si/DNA-ThT -Calculate concentrations of gold, DNA, and Beacon
|