User:Allison K. Alix/Notebook/Thesis Research/2013/09/17

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(FCS Curves)
Current revision (09:24, 24 September 2013) (view source)
(Attaching MB to Au@SiO2-DNA)
 
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* Run AuNp@SiO2/DNA_MB Samples
* Run AuNp@SiO2/DNA_MB Samples
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==FCS Curves==
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==FCS Curves Antibody-Alexa 532==
[[Image:10nM_Antibody-Alexa_532_pic.png‎]]
[[Image:10nM_Antibody-Alexa_532_pic.png‎]]
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[[Image:100pM_Antibody-Alexa_532_pic.png]]
[[Image:100pM_Antibody-Alexa_532_pic.png]]
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[[Image:10pM_Antibody-Alexa_532_pic2.png]]
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[[Image:1pM_Antibody-Alexa_532_pic.png]]
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==Attaching MB to Au@SiO2-DNA==
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1) Add 13.3 uL 7.61uM MB to 100uL Au@SiO2-DNA
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2) Hybridize by heating for 20 min @ 75C
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3) Slowly cool to room temperature.
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4) Prepare 5X, 10X, and 100X dilutions in PBS
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5) Run FCS Measurements on each. If signal is seen with the least concentrated solution, prepare 1000X and 10,000X dilutions as well.
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==FCS Curves Au@SiO2-DNA-MB==
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[[Image:2x.png‎]]
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[[Image:10X.png‎]]
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[[Image:100X.png‎]]
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[[Image:1000X.png‎]]
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[[Image:10000X.png‎]]
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==Next Up==
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-Run Au/DNA-MB without silica coating
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-Run Au@Si/DNA-ThT
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-Calculate concentrations of gold, DNA, and Beacon
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Current revision

Project name Main project page
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To Do

  • Run Antibody-Alexa 532 Samples
  • Run AuNp@SiO2/DNA_MB Samples

FCS Curves Antibody-Alexa 532

Image:10nM_Antibody-Alexa_532_pic.png‎

Image:1nM_Antibody-Alexa_532_pic.png‎

Image:100pM_Antibody-Alexa_532_pic.png

Image:10pM_Antibody-Alexa_532_pic2.png

Image:1pM_Antibody-Alexa_532_pic.png

Attaching MB to Au@SiO2-DNA

1) Add 13.3 uL 7.61uM MB to 100uL Au@SiO2-DNA

2) Hybridize by heating for 20 min @ 75C

3) Slowly cool to room temperature.

4) Prepare 5X, 10X, and 100X dilutions in PBS

5) Run FCS Measurements on each. If signal is seen with the least concentrated solution, prepare 1000X and 10,000X dilutions as well.

FCS Curves Au@SiO2-DNA-MB

Image:2x.png‎

Image:10X.png‎

Image:100X.png‎

Image:1000X.png‎

Image:10000X.png‎

Next Up

-Run Au/DNA-MB without silica coating

-Run Au@Si/DNA-ThT

-Calculate concentrations of gold, DNA, and Beacon



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